The colorimetric assay of diamine oxidase activity with high sensitivity based on calixarene derivative-capped gold nanoparticles
aCenter for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, China
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bShanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China
cInstitute of General Education, Pusan National University, Busan 609-735, Republic of Korea
dDepartment of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China
Diamine oxidase (DAO) is involved in regulating ingested or endogenous histamine, several types of human cancer, and the cell mass during embryonic development. Furthermore, DAO is a potential indicator of intestinal mucosa damage in inflammatory and neoplastic diseases or after chemotherapy. Therefore, developing a biosensor is urgently needed to assay DAO activity. In this work, a simple but sensitive colorimetric biosensor is developed for DAO assay and inhibitor screening. The biosensor is based on host-guest interactions between the amine group on the terminal of 1,6-hexanediamine (HMD) and p-sulfonatocalixarene (pSC6). These interactions may aggregate pSC6 modified gold nanoparticles (AuNPs) and induce the corresponding color change of the test solution. Specifically, AuNP aggregation is affected because coordination reactivity does not exist between oxidized HMD and pSC6 during HMD oxidation by DAO. Therefore, a simple colorimetric method with high sensitivity for the assay of DAO activity is proposed. A linear relationship is presented under optimized experimental conditions in a range of 0.15 mU mL−1 to 4.5 mU mL−1 with the lowest detection limit of 0.062 mU mL−1. Moreover, the inhibition effect of guanidine on DAO activity is tested with the IC50 value of 2.4 μM using the proposed biosensor. Therefore, this biosensor has great potential not only for the detection of DAO activity but also for inhibitor screening in future.
Associations Between Migraine, Celiac Disease, Non-Celiac Gluten Sensitivity and Activity of Diamine Oxidase
Download pdf K Griauzdaitė1, K Maselis2, A Žvirblienė1, A Vaitkus1, D Jančiauskas1, I Banaitytė-Baleišienė1, L Kupčinskas1, D Rastenytė1 Medical Hypotheses...