Respective leaders in fermentation science and DAO-based medical nutrition join together to focus on innovative enzyme-based solutions for the human nutrition & health market.
PETERBOROUGH, U.K. 26 may 2021 – AB Biotek, a global business division of Associated British Foods plc – today announces that it has acquired DR Healthcare, a biomedical company focused on the dietary management of chronic diseases based in Barcelona, Spain.
This acquisition enables AB Biotek to boost its growing portfolio of differentiated microbiome modulating solutions for human nutrition and health. The acquisition adds diamine oxidase (DAO), an important intestinal enzyme, that will expand AB Biotek’s already strong commercial portfolio of probiotic yeast strains.
DAO, naturally secreted by the human body, is widely known as a critical enzyme at the core of reducing symptoms of many of today’s ailments, including migraine, food intolerances and attention deficit hyperactivity disorder, prevalent in millions of people around the world.
Juanjo Duelo, the founder of DR Healthcare, will remain with the business.
“With the acquisition of DR Healthcare, we now have the next stepping stone in shaping our science-based organization to serve the fast-growing human nutrition and health space,” said Gerald Dard, global managing director, AB Biotek. “DR Healthcare has a strong track record in this area, and we welcome our new colleagues who are equally passionate about the health and wellness of today’s consumers.”
“I am very excited about this new chapter as our companies have many shared values, from advanced health solutions and deep science to an extreme passion for what we do,” said Juanjo Duelo. “The future is bright under the good ownership of AB Biotek, and the timing is perfect to bring new, innovative technologies to improve the wellbeing of those people who suffer from various pathologies associated with the deficiency of DAO.”
About AB Biotek
AB Biotek contributes to the success of customers through the delivery of customized fermentation-based, sustainable solutions containing proprietary and/or purposefully sourced microorganisms to created differentiated food, beverage, health & nutrition business opportunities. Science and advanced microorganism fermentation technology are core enablers. AB Biotek has commercial scale production and research development facilities located around the world. For more information about our product applications and unmatched technical service capabilities, please visit www.abbiotek.com.
About DR Healthcare
Founded in Barcelona, Spain, in 2007, DR Healthcare is a biomedical company specialized in medical nutrition and dedicated to the research, development, innovation, marketing and licensing of new nutraceuticals, functional foods, active ingredients and customized solutions. Our medical nutrition products are aimed at dietary management of chronic diseases, physiological dysfunctions and/or metabolic diseases, mainly associated with metabolic disorders caused by a deficiency of the intestinal enzyme diamine oxidase (DAO). DR Healthcare produces DAO for both human and animal use and offers proprietary solutions for the food, pharmaceutical, veterinary and animal health industries. More information is available at www.dr-healthcare.com/en.
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The Coronavirus has altered our lives. And it will also alter this Christmas, which will undoubtedly be different. Still, some things are not going to change compared to the Christmas holidays of other years: emotions increase, alcohol is exceeded, meals are exaggerated and, consequently, many people will have heavy digestion, flatulence, a feeling of bloating, contractures, hangover, headache and even migraine.
Histamine in Christmas foods
And it is not only COVID-19 alters Christmas. A molecule present in most “Christmas” foods is related with all these symptoms and also alters our lives. Is the histamine, a molecule necessary for life but that in excess becomes “perverse”. An amine that is especially present and abundant in the foods consumed at Christmas parties: by tradition, Christmas is accompanied by certain foods with a high concentration of histamine such as canapes, appetizers with sausages, smoked meats, assorted sauces such as the bechamel of the cannelloni or the tartar sauce of the fish; seafood, canned foods such as anchovies, spices such as curry or cinnamon, nougats, polvorones or roscón de reyes, cava, champagne and other alcoholic beverages.
DAO enzyme to avoid its effects
The Diamine Oxidase or DAO enzyme is responsible for metabolizing or degrading the histamine ingested through these foods. However, in these days of dietary excesses DAO activity is saturated or even inhibited by alcohol, so our best recommendation is to prevent the appearance of these symptoms by previously supplementing Christmas menus with DAO enzyme.
This measure is also advisable for those people who spend Christmas at home having tested positive for their last coronavirus test. Published studies indicate that SAR COV-2 infection causes a release of cytokines from mast cells, accompanied by a release of endogenous histamine, which may justify the triggering of some of the symptoms, so it is advisable to control the entry routes of dietary histamine or guarantee its degradation by taking DAO preventively to avoid further accumulation in plasma.
Our DAO products
DAOfood® and DAOfood® Plus are biological DAO enzyme supplements that increase the degradation capacity of dietary histamine. Its intake prevents the absorption and accumulation of circulating histamine and also counteracts the release of endogenous histamine as a reaction of the body when it comes into contact with the virus.
DR Healthcare wishes you a Happy Holidays!
Kritas, S. K., Ronconi, G., Caraffa, A., Gallenga, C. E., Ross, R., & Conti, P. (2020). Mast cells contribute to coronavirus-induced inflammation: new anti-inflammatory strategy. Journal of biological regulators and homeostatic agents, 34(1), 10.23812/20-Editorial-Kritas.
On October 23rd the magazine SABER VIVIR tve published an article about the DAO deficiency, its causes and management called “Migraine, itching, stomach pain… This is how DAO deficiency affects you”.
It is signed by the journalist specialized in health Eva Mimbrero and has the advice of the allergist Dr. Pedro Ojeda, co-director of the clinic Ojeda of Asthma and Allergy and a member of the International Society of Deficit of DAO.
To access the original article click here clic here.
What is histamine?
The article begins by explaining what the histamine molecule is, what its sources are in the organism (since it is produced and stored in certain cells, and can also be acquired through food in the case of a deficit of DAO) and what physiological functions it performs, among which are the secretion of gastric juices, processes of the immune system, neurotransmission in the nervous system, among others.
Which systems are affected?
After introducing the role of histamine in the body, the deficit of DAO and its symptoms are discussed. This consists of an alteration in the metabolism of histamine that comes from food, this alteration being caused by the deficit of the enzyme Diamino Oxidase.
Among its symptoms at the neurological level is migraine (87% of people who suffer from migraine have a deficit of DAO, according to a clinical study conducted in Spain), at the skin level itching, redness, emergence of beans, hives and angioedema, at intestinal level gastric pain, cramps, gas or diarrhea, at gynecological level menstrual pain, at cardiac level palpitations, arrhythmias or tachycardias, and at respiratory level rhinitis and the feeling that it is difficult to breathe.
Which are the causes?
Before going on to talk about methods for the dietary management of the symptoms of DAO deficiency, it is emphasized that DAO deficiency is not an allergic response but that its effects and control are more prolonged over time, being more durable and less instantaneous processes.
Finally, the article identifies the main causes of DAO deficiency, among which are the genetic alteration in the individual’s DAO enzyme, certain inflammatory intestinal disorders that alter the intestinal mucosa where DAO is produced (such as celiac disease, Crohn’s disease or ulcerative colitis), the consumption of certain drugs that inhibit the enzyme, or the consumption of alcoholic beverages, since alcohol also acts as a potent inhibitor of the enzyme.
How to handle it?
Once these causes have been identified, it ends up talking about the management of the symptoms, in which its three fundamental pillars are the diagnosis of the specific cause in each patient, avoiding the consumption of alcohol and certain foods rich in histamine, as well as keeping a medical control of the drugs that are consumed, and finally following a dietary supplementation of diamond oxidase.
To access the original article click here clic here.
Associations Between Migraine, Celiac Disease, Non-Celiac Gluten Sensitivity and Activity of Diamine Oxidase
Background and pilot study: Recent reports reveal a close relationship between migraine and gastrointestinal disorders (GI), such as celiac disease (CD) and non-celiac gluten sensitivity (NCGS). CD is a genetic autoimmune disorder, which affects the mucosa of the small intestine. Gluten, found in various grains, not only plays a major role in the pathophysiology of CD and NCGS, but also aggravates migraine attacks. Another common food component, which can induce migraine headaches, is histamine. Diamine oxidase (DAO) is an enzyme, which degrades histamine. Reduced activity of DAO means reduced histamine degradation, which can cause histamine build-up and lead to various symptoms, including headaches and migraine. In this paper we propose a hypothesis, that in pathogenesis of migraine, low serum DAO activity is related to CD and NCGS. We also conducted our own pilot study of 44 patients with severe migraine in efforts to evaluate the co-presence of decreased serum DAO activity and celiac disease/NCGS in patients. 44 consecutive migraine patients were divided into 2 groups: decreased DAO activity (group 1; n = 26) and normal DAO activity (group 2; n = 18). All patients were screened for celiac disease. The diagnosis of NCGS was made after exclusion of CD, food allergies and other GI disorders in the presence of gluten sensitivity symptoms. Furthermore, dietary recommendations were given to all participants and their effects were assessed 3 months after the initial evaluation via the MIDAS (Migraine Disability Assessment) questionnaire.
Results and conclusions: Only 1 patient fit the criteria for celiac disease, rendering this result inconclusive. Pathological findings of the remainder of patients were attributed to NCGS (n = 10). 9 of 10 patients with NCGS belonged to the decreased serum DAO activity group (group 1; n = 26), suggesting a strong relationship between reduced serum DAO activity and NCGS. MIDAS questionnaire revealed, that patients with decreased serum DAO activity were more severely impacted by migraine than those with normal DAO activity, and this remained so after our interventions. Dietary adjustments significantly reduced the impact of migraine on patients’ daily activities after 3 months in both groups. We argue, that migraine, celiac disease and NCGS may benefit from treatment with a multidisciplinary approach, involving neurologists, gastroenterologists and dietitians.
We live in a time of real uncertainty with the worldwide spread of COVID-19 and anxiety levels are high. It is important to remain calm, listen and apply the advice of the Health Authority.
Considering that the activities of DR Healthcare are focused on the study of histamine, DAO deficiency and histamine intolerance, it is worth mentioning recent publications that support the fact that mast cell activation and histamine release can contribute to the inflammation associated with SARS COV-2 infection (new strain of coronavirus that causes COVID-19) .
Coronavirus infection (regardless of the various types of coronavirus) is primarily attacked by immune cells, including mast cells (MC), which are found in the submucosa of the respiratory tract and in the nasal cavity, representing a protective barrier against microorganisms. Viruses activate mast cells that release early inflammatory chemical compounds such as histamine and protease (1). In people with DAO deficiency who, due to this condition, have high levels of circulating histamine, a greater histamine accumulation caused by the aforementioned virus-activated mastocytosis, may aggravate the inflammatory process generated by the infection.
Under normal conditions, when histamine levels rise above a certain threshold, the enzymes responsible for its metabolism, specifically DAO (Diamino Oxidase) and, to a lesser extent, histamine N-methyl transferase (HNMT), rapidly degrade the excess this amine. However, when the degradation rate is insufficient to cope with the excess, the total level of histamine in the body rises. At a certain critical level, signs and symptoms occur as a result of histamine coupling to its receptors in specific cells.
Histamine is a mediator that intervenes in the inflammation of the immune system by activating intracellular pathways that induce the production of cytokines in the lungs. Cytokines are signaling proteins that regulate interactions of cells of the immune system, thus producing an amplification of inflammatory reactions.
Based on the aforementioned studies and the pathophysiology of the disease, it is important to maximize the basic recommendations to protect against COVID-19, avoiding an accumulation of histamine in people with DAO deficiency and / or mastocytosis. Since inflammation is a concern in the severity of the disease caused by SARS COV-2, it is recommended to reduce the consumption of foods high in histamine in the diet and take supplements that allow maintaining a balance between endogenous and exogenous histamine, avoiding an excess of histamine in the body, and therefore reducing the mast cell / histamine load.
Thus, in addition to the basic general guidelines to prevent the spread of COVID-19 in the entire population, there are a number of added recommendations to consider in people with DAO deficiency and / or intolerance to histamine:
-Avoid eating foods with a high concentration of histamine or other biogenic amines that compete for the DAO enzyme. These include, for example, raw, pasteurized milk and dairy products; citrus, strawberries and banana; seafood, blue fish and fish sauces; the raw sausage, such as sausage, chorizo or bacon; some vegetables, such as tomato, spinach or eggplant, as well as alcoholic beverages.
-Oral supply of DAO enzyme to prevent systemic absorption of exogenous or dietary histamine. This facilitates the metabolism of food histamine locally in the small intestine, restoring the natural balance of histamine and DAO.
–DAOfood® Plus contains the DAO enzyme that complements the body’s own enzyme and increases the degradation capacity of exogenous histamine. It also contains Quercetin that acts as an adjuvant against endogenous histamine. Its intake prevents the absorption and accumulation of circulating histamine and therefore counteracts the release of endogenous histamine as a reaction of the body when it comes into contact with the virus.
It is important to note that because SARS COV-2 is classified as a new strain of coronavirus, there is limited scientific evidence available on its precise mode of action and infection. Therefore, what is indicated in this article is based on what we know so far and on the importance of protecting against the virus, while maintaining control of mastocytosis and DAO deficiency.
The DR Healthcare team is at your entire disposal in case of concern about the implications of COVID-19 in people with DAO deficiency.
1.- Kritas, S. K., Ronconi, G., Caraffa, A., Gallenga, C. E., Ross, R., & Conti, P. (2020). Mast cells contribute to coronavirus-induced inflammation: new anti-inflammatory strategy. Journal of biological regulators and homeostatic agents, 34(1), 10.23812/20-Editorial-Kritas.
Given the difficult scenario that we are facing caused by the Coronavirus pandemic in general and after the State of Alarm decreed by the Spanish government in particular, DR Healthcare has implemented a safety and business continuity protocol that guarantees the provision of our services, the supply of the active ingredient, and, safeguard the health of all our collaborators and customers.
In this sense, we want to indicate that:
• The staff in the areas of administration, customer service and patient care operate in telework mode to meet themobility and contact requirements, guaranteeing the maintenance of their normal operation and service.
• Laboratory and R&D personnel who cannot carry out their activity remotely, following the security protocol, have established rotating work shifts to avoid human contact.
• The staff of the enzyme production plant has also implemented an intensive schedule protocol with rotating shifts that prevents human contact andensures non-interruption of the DAO enzyme production process.
Likewise, our suppliers and logistics network have adapted their processes to the current situation, ensuringthe safety and continuity of the supply chain of both the active ingredient and finished products up to the final customer, whether national or international.
We thus contribute to the containment of the disease and comply with the measures required by the competent authorities, guaranteeing our best service.
The DR Healthcare team is at your disposal for any additional information you may require.
CEO DR Healthcare
You can download the supplement in pdf clicking in the image below or through here. (LANGUAGE: SPANISH)
el farmacéutico.es publishes its supplement nº 585 entitled “Migraine and DAO deficiency (Diamine Oxidase)”.
In this publication the scientific and clinical bases of migraine caused by DAO deficiency are exposed and the diagnostic methods and forms of migraine management caused by this metabolic alteration are shown. Products that can be found in pharmacies for the management and diagnosis of migraine caused by DAO deficiency are also shown.
The supplement has been prepared by the PhD. Medical Writer Antoni X. Torres-Collado and Eva Morán-Salvador, edited by MAYO editions and published on March 2 – 2020.
Contents of supplement nº585 “Migraine and DAO deficiency (Diamine Oxidase)”:
- What is migraine?
2. Symptomatology and diagnosis
3. Management and treatment of migraine
- What happens in migraine?
1. Phases of the migraine crisis
2. Phystopathological mechanisms
3. What is histamine?
- What is Diamino Oxidase?
- DAO and migraine deficit
1. pregnancy and migraine
2. associated symptoms (migraine comorbidity)
4. Management of Migraine due to DAO deficiency
- How to manage migraine due to DAO deficiency from the pharmacy?
1. DAO supplementation in the patient with migraine: food for special medical uses
Publication of the article “Do you know the difference between DAOfood and Migrasin? In the blog about health and wellness tips, novel and natural beauty treatments dosfarma.com. Language: Spanish.
This paper informatively discusses the role of food histamine and the enzyme diamino oxidase (DAO) in DAO deficiency and its associated pathologies. It also shows us how the DR Healthcare company is one of the pioneer companies in the investigation of this widespread metabolic disorder and informs us of the composition and indications of its DAOfood and Migrasin products. So, if you don’t know the differences between DAOfood and Migrasin, this article will show you what to take in each moment properly.
DAOfood is indicated for the treatment and prevention of gastrointestinal, cutaneous and muscular disorders and pathologies. Being its main component the enzyme diamino oxidase (DAO).
Migrasin, containing DAO enzyme, group B vitamins (B6, B9 and B12) and caffeine, is indicated for the treatment and prevention of migraine and muscle headaches.
To access the article in dosfarma.com click here.
Association of Diamine oxidase (DAO) variants with the risk for migraine from North Indian population
Association of Diamine oxidase (DAO) variants with the risk for migraine from North Indian population
Sukhvinder Kaura,⁎, Arif Alib, Yaser Siahbalaeic, Uzair Ahmadc, Fazila Nargisc, A.K. Pandeyd,
a UGC-PDF, Gene Expression Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India
b UGC-BSR-FF, Department of Biosciences, Jamia Millia Islamia, New Delhi, India
c Department of Biosciences, Jamia Millia Islamia, New Delhi, India
d Head, Department of Physiology, ESIC Medical College & Hospital, Faridabad, India
e NC Medical College & Hospital, Panipat, India
A B S T R A C T
Background: Migraine is a common neurovascular disorder affected by various levels of neurotransmitters. Low histamine metabolism is also related with pathophysiology of migraine. As diamine oxidase (DAO) gene variants are linked with higher levels of histamine in migraine patients, we investigated the possible relationship of two variants rs2052129 and rs10156191of this gene with migraine risk in North Indian population.
Methods: A case-control study for 250 migraine patients and 250 matched healthy controls was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results: We found statistically significant differences in allelic frequencies of rs2052129 (p = .009, OR = 1.462; 95% CI: 1.098–1.947) and rs10156191 (p = .019, OR = 1.430; 95% CI: 1.060–1.928) variants in DAO gene. For rs1015691, we were able to show statistically significant association at all genotypic, dominant and allelic levels in both MA (for T allele, p = .020; OR = 1.662, 95% CI: 1.083–2.551) as well as in female subgroup (for T allele, p = .025, OR = 1.460; 95% CI: 1.049–2.033). But no such significant association was found in clinical sub grouping of migraine in rs2052129 as p > .05. However in gender analysis, protective effect of T allele in male migraine patients for rs2052129 (OR < 1) was found.
Conclusions: Our findings clearly indicated that female patient with rs10156191T allele and in MA subgroup showed an increased risk for migraine. Our data also indicated that rs2052129T variant showed a significant role in migraine susceptibility of this population.
Impaired resolution of wheals in the skin prick test and low diamine oxidase blood level in allergic patients
Aneta Wagner1, Krzysztof Buczyłko2, Hanna Zielińska-Bliźniewska1, Waldemar Wagner3
1Allergology and Respiratory Rehabilitation Department, Medical University of Lodz, Lodz, Poland
2NZOZ Allergology Center, Lodz, Poland
3Laboratory of Cellular Immunology, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland
Adv Dermatol Allergol 2019; XXXVI (5): 538–543
Introduction: Histamine is the major mediator of IgE- and non-IgE-mediated allergic reactions upon allergen or
hapten contact. Reduced histamine degradation capacity was associated with atopic eczema as well as with nonimmunological
histamine intolerance. Higher blood serum histamine level concomitant with decreased intestinal
diamine oxidase activity were observed in patients with food allergy.
Aim: To evaluate the relationship between patients’ blood diamine oxidase (DAO) activity/histamine status and
their reactivity to time-resolved histamine skin prick test in respect to vulnerability to allergic diseases.
Material and methods: Fifty-three patients were examined with skin prick tests (SPT) and patch tests for suspected
presence of either IgE- or non-IgE-mediated allergy. All individuals were skin prick tested with histamine and the
resolution of the wheal was monitored for 50 min. Blood DAO activity and histamine concentration were measured
with a radio-extraction radioimmunoassay.
Results: Time-resolved histamine skin prick testing revealed presence of wheals which were 35% larger in diameter
in 47% of examined subjects at 20 min of the test. These patients exhibited significantly compromised time-course
wheal resolution (wheal ≥ 3 mm at 50 min) compared to a group of patients with the normal-rate of wheal resolution
(wheal = 0 mm at 50 min). Within a group of subjects exhibiting impaired wheal resolution, 61% of patients
were diagnosed allergic compared to 50% in a group of patients with a normal rate of wheal resolution. Finally,
allergic patients were characterized by a significantly lower DAO activity and higher histamine content compared
to healthy subjects.
Conclusions: The results of this study indicate that patients with IgE- or non-IgE-mediated allergy are likely to
have low DAO blood activity and may concomitantly suffer from histamine intolerance. Furthermore, our results
suggest that allergic patients are more likely to develop an excessive SPT reaction. Our results emphasize caution
in interpretation of the SPT results in allergic patients with diagnosed histamine intolerance or histamine/DAO