Association of Diamine oxidase (DAO) variants with the risk for migraine from North Indian population
Association of Diamine oxidase (DAO) variants with the risk for migraine from North Indian population
Sukhvinder Kaura,⁎, Arif Alib, Yaser Siahbalaeic, Uzair Ahmadc, Fazila Nargisc, A.K. Pandeyd,
Balkirat Singhe
a UGC-PDF, Gene Expression Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India
b UGC-BSR-FF, Department of Biosciences, Jamia Millia Islamia, New Delhi, India
c Department of Biosciences, Jamia Millia Islamia, New Delhi, India
d Head, Department of Physiology, ESIC Medical College & Hospital, Faridabad, India
e NC Medical College & Hospital, Panipat, India
A B S T R A C T
Background: Migraine is a common neurovascular disorder affected by various levels of neurotransmitters. Low histamine metabolism is also related with pathophysiology of migraine. As diamine oxidase (DAO) gene variants are linked with higher levels of histamine in migraine patients, we investigated the possible relationship of two variants rs2052129 and rs10156191of this gene with migraine risk in North Indian population.
Methods: A case-control study for 250 migraine patients and 250 matched healthy controls was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results: We found statistically significant differences in allelic frequencies of rs2052129 (p = .009, OR = 1.462; 95% CI: 1.098–1.947) and rs10156191 (p = .019, OR = 1.430; 95% CI: 1.060–1.928) variants in DAO gene. For rs1015691, we were able to show statistically significant association at all genotypic, dominant and allelic levels in both MA (for T allele, p = .020; OR = 1.662, 95% CI: 1.083–2.551) as well as in female subgroup (for T allele, p = .025, OR = 1.460; 95% CI: 1.049–2.033). But no such significant association was found in clinical sub grouping of migraine in rs2052129 as p > .05. However in gender analysis, protective effect of T allele in male migraine patients for rs2052129 (OR < 1) was found.
Conclusions: Our findings clearly indicated that female patient with rs10156191T allele and in MA subgroup showed an increased risk for migraine. Our data also indicated that rs2052129T variant showed a significant role in migraine susceptibility of this population.
According to a study the 90% of migraines can be treated with the enzyme DAO – Interview of RTVE to Juanjo Duelo, CEO of DR Healthcare
7/3/2013 – interview to Juanjo Duelo in rtve 24h. Language: Spanish
These last hours has been spoken about migraines, from which has been made public that the fifteen percent of the world’s population suffers from these tremendous headaches that go beyond a headache. Juanjo Duelo, in addition to suffering from migraines, directs a research team that assures to be able to determine the causes of migraines and most importantly, their solution.
Conformational Design and Characterisation of a Truncated Diamine Oxidase from Arthrobacter globiformis
Conformational Design and Characterisation of a Truncated Diamine Oxidase from Arthrobacter globiformis
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Abstract
A functional mini protein can be developed by miniaturising its size. The minimisation technique provides an excellent model system for studying native enzymes, especially in creating an alternative novel biocatalyst. Miniaturised proteins may have enhanced stability, a crucial characteristic for large-scale production and industrial applications. In this study, a huge enzyme molecule, known as diamine oxidase (DAO, comprising 700 amino acids), was selected to undergo the process.
Methods
Results
The colorimetric assay of diamine oxidase activity with high sensitivity based on calixarene derivative-capped gold nanoparticles
The colorimetric assay of diamine oxidase activity with high sensitivity based on calixarene derivative-capped gold nanoparticles
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Abstract
Diamine oxidase (DAO) is involved in regulating ingested or endogenous histamine, several types of human cancer, and the cell mass during embryonic development. Furthermore, DAO is a potential indicator of intestinal mucosa damage in inflammatory and neoplastic diseases or after chemotherapy. Therefore, developing a biosensor is urgently needed to assay DAO activity. In this work, a simple but sensitive colorimetric biosensor is developed for DAO assay and inhibitor screening. The biosensor is based on host-guest interactions between the amine group on the terminal of 1,6-hexanediamine (HMD) and p-sulfonatocalix[6]arene (pSC6). These interactions may aggregate pSC6 modified gold nanoparticles (AuNPs) and induce the corresponding color change of the test solution. Specifically, AuNP aggregation is affected because coordination reactivity does not exist between oxidized HMD and pSC6 during HMD oxidation by DAO. Therefore, a simple colorimetric method with high sensitivity for the assay of DAO activity is proposed. A linear relationship is presented under optimized experimental conditions in a range of 0.15 mU mL−1 to 4.5 mU mL−1 with the lowest detection limit of 0.062 mU mL−1. Moreover, the inhibition effect of guanidine on DAO activity is tested with the IC50 value of 2.4 μM using the proposed biosensor. Therefore, this biosensor has great potential not only for the detection of DAO activity but also for inhibitor screening in future.
DOI:10.1039/C7AY00227K
Progress on the deficit of the enzyme DAO
Read the original article in LA VANGUARDIA
New research led by Dr. Ramon Tormo, Chief of Gastroenterology and Nutrition Hospital Chiron Barcelona, attributed migraine to reaffirm deficit DAO enzyme.
Plasma diamine oxidase activity is a useful biomarker for evaluating gastrointestinal tract toxicities during chemotherapy with oral fluorouracil anti-cancer drugs in patients with gastric cancer.
Plasma diamine oxidase activity is a useful biomarker for evaluating gastrointestinal tract toxicities during chemotherapy with oral fluorouracil anti-cancer drugs in patients with gastric cancer.
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Author information
- 1 Department of Surgery, Kochi Medical School, Nankoku, Japan. tsutomun@kochi-u.ac.jp
Abstract
OBJECTIVES:
Diamine oxidase (DAO) is an enzyme that catalyzes oxidation and is highly active in the mature upper villus cells of the intestinal mucosa. This study sought to evaluate plasma DAO activities during adjuvant chemotherapy in patients with gastric cancer.
METHODS:
We investigated 20 patients with gastric cancer who were treated with oral fluorouracil anti-cancer drugs as adjuvant chemotherapy. Plasma DAO activity was measured in all patients before chemotherapy and at 2, 4 and 6 weeks after the start of chemotherapy, and quality of life was evaluated simultaneously.
RESULTS:
The median DAO activity after 4 weeks of chemotherapy was significantly decreased compared to the pre-chemotherapy levels (6.6 vs. 7.5 U/l; p = 0.038). The changes in the rate of DAO activity at 2 and 6 weeks following the start of chemotherapy in patients with gastrointestinal tract toxicity were significantly lower than in those without toxicity (p =0.021 and 0.047, respectively). The patient cohort showed a slightly positive correlation between DAO activity and global health status and a negative correlation between DAO activity and appetite loss.
CONCLUSIONS:
Plasma DAO activities may be useful for monitoring and evaluating gastrointestinal tract toxicities induced by adjuvant chemotherapy with oral fluorouracil in patients with gastric cancer.
Copyright © 2012 S. Karger AG, Basel.
- PMID: 22433290
- DOI: 10.1159/000336799